Coding

Part:BBa_K592001:Design

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-21)

CcaS, green light sensor from Synechocystis sp. PCC6803


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 606
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2252
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1270
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This DNA was extracted from Tabor's pJT122 plasmid. Two illegal internal restriction sites had to be removed in order to allow BioBrick-compatibility. The removed sites are: A1282C and A1284C to remove EcoRI site, and T1383C to remove SpeI site. Furthermore, while removing the EcoRI site, G1278T was also done to enhance codon usage (GGG to GGT).

Source

Synechocystic sp. PCC6803, pJT122 plasmid of Jeffrey Tabor

References

Tabor, J. J., Levskaya, A., and Voigt, C. A. (2011). Multichromatic control of gene expression in Escherichia coli. J. Mol. Biol. 405, 2, 315–324.